MicroLab
Andre Willers
9 Feb 2015
This is not medical advice .
Synopsis :
Skin Patches to train the immune system .
Discussion :
1.A microsize tangle of stalks shelters a MicroLab to
fast-test various biohazard solutions .
See Appendix C for present applications .
See http://andreswhy.blogspot.com/2014/09/prodigies-update-i.html
for nano applications .
A MicroLab enables a quick-and-dirty application approach
using kitchen equipment without too big a hazard .
See Appendix A , B for how it works .
2.You want low quality teadust or stinging nettle tea bag
for the small micronsize
3.Infuze it with the payload
4. Heat it to 1/3(37-20) +20 ~ 25 celsius . You need an
optimization of chemical activity and transport across the skin .This is
temperature dependant (general theory of optimals) .
5.The Formic acid renders the the skin permeable , and the
temperature difference drives the process from the body into the patch .Here
the various systems interact as described in Appendix A,B,C .
6.Leave for at least 10 minutes (for long-term
potentionation of dendritic immune cells to occur) http://en.wikipedia.org/wiki/Long-term_potentiation
7.The Trick !
Now slap on a heated pad just below Pasteurization
temperature but over body temp to drive the reaction products back into the
body past the skin barrier . Another ten
minutes .
A bit less than 63 Celsius (145F) . This is about the tolerable temperature for
hot drinks .So now you know .
This is quite finicky : http://www.engineeringtoolbox.com/pasteurization-methods-temperatures-d_1642.html
63ºC (145ºF) 30
minutes
72ºC (161ºF) 15
seconds
8. Buccal Transforms .
The inside skin of the gastro-intestinal tract is hard to
reach by this method .
But there are evolved shortcuts , clustered around the
descending duodenal in humans .
The general method is to soak the stinging nettle teabag in
the payload , suck it for ten minutes , spitting out any liquid . It is important
that nothing be swallowed .
Then rinse , swill and spit with 63C liquid for 10 minutes .
That’s it . Reprogramming is idiosyncratic . That’s fancy
speak for that no one knows . Try it .
9.Diabetes II and RLS (related)
The payload is simple sugar .
See a simpler form in http://andreswhy.blogspot.com/2015/01/rls-stopper.html
The method should work for Diabetes II , because Gastric
bypasses work .
10. Fasting past the 12 hour mark .
This works from personal experience .
Why ? More than the dopamine reward response is involved,
but I don’t have the facilities to find out . Frustrating !
11.Eating disorders .
One can intuit that this involves temperatures , micro sizes
of foodstuffs and the orders of ingestion
messing up the reward systems .
The microwave has to bear some scrutiny .
Always drinking sugary liquids with suspended micron-structures
at 63C is going to screw up your reward systems .
Especially if milk is involved.
12. Idoru-Mama
An HyperImage-Mama as perceived by the body .
That extra-latte heated and reheated is setting you up for anorexia
, bulimia , binges , and later diabetes .
The prevention is absurdly simple :
As soon as it is cool enough to sip (ie about 63C) , spit in
it . That’s it . As it cools down further , the processes described above take
place . Over time it adds up .
Who would have thought ?
Andre .
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Appendix A .An indication of size :
|
Particle
|
Particle Size
(microns) |
|
Tea Dust
|
8 - 300
|
|
Coffee
|
5 - 400
|
|
Cayenne Pepper
|
15 - 1000
|
|
Ginger
|
25 - 40
|
|
Starches
|
3 - 100
|
|
Mustard
|
6 - 10
|
|
Gelatin
|
5 - 90
|
|
Milled Flour,
Milled Corn
|
1 - 100
|
|
Corn Starch
|
0.1 - 0.8
|
|
Sea Salt
|
0.035 - 0.5
|
|
Yeast Cells
|
1 - 50
|
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Appendix B
Synopsis :
Formic acid opens the
Blood Brain Barrier(BBB) . This enables the immune system to interact with
brain cells . Only to be used in very controlled circumstances .
Discussion :
1.The Blood Brain
Barrier evolved from skin .
2.Formic acid bypasses
skin .
Synopsis:
Commercially
available desensitization technologies are now available for Milk , Peanut and
DustMite allergies .
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Appendix C
3-D
vaccine spontaneously assembles to pack a powerful punch against cancer,
infectious diseases
Date:
February 9, 2015
Source:
National Institute of
Biomedical Imaging and Bioengineering
Summary:
Researchers have
developed a novel 3-D vaccine that could provide a more effective way to
harness the immune system to fight cancer as well as infectious diseases. The
vaccine spontaneously assembles into a scaffold once injected under the skin
and is capable of recruiting, housing, and manipulating immune cells to
generate a powerful immune response. The vaccine was recently found to be
effective in delaying tumor growth in mice.
3-D vaccine consists
of many microsized, porous silica rods that spontaneously assemble into a
haystack formation after being injected under the skin. Image taken with
polychromatic scanning electron microscope.
Credit: James C.
Weaver, Wyss Institute
NIBIB-funded researchers have developed a
novel 3D vaccine that could provide a more effective way to harness the immune
system to fight cancer as well as infectious diseases. The vaccine spontaneously
assembles into a scaffold once injected under the skin and is capable of
recruiting, housing, and manipulating immune cells to generate a powerful
immune response. The vaccine was recently found to be effective in delaying
tumor growth in mice.
Related Articles
·
T cell
"This vaccine is
a wonderful example of applying biomaterials to new questions and issues in
medicine," says David Mooney, Ph.D., a professor of bioengineering at
Harvard University in the School of Engineering and Applied Sciences, whose lab
developed the vaccine. The project was co-led by Jaeyun Kim, Ph.D. and Aileen
Li, a doctoral student in the Mooney lab. Their findings were published in the
December 8, 2014 issue of Nature Biotechnology.
Cancer vaccines
Cancer cells are
generally ignored by the immune system. This is because -- for the most part --
they more closely resemble cells that belong in the body than pathogens, such
as bacterial cells or viruses. The goal of cancer vaccines is to provoke the immune
system to recognize cancer cells as foreign and attack them.
One way to do this is
by manipulating dendritic cells, the coordinators of immune system behavior.
Dendritic cells constantly patrol the body, sampling bits of protein found on
the surface of cells or viruses called antigens. When a dendritic cell comes in
contact with an antigen that it deems foreign, it carries it to the lymph
nodes, where it instructs the rest of the immune system to attack anything in
the body displaying that antigen.
Though similar to
healthy cells, cancer cells often display unique antigens on their surface,
which can be exploited to develop cancer immunotherapies. For example, in
dendritic cell therapy, white blood cells are removed from a patient's blood,
stimulated in the lab to turn into dendritic cells, and then incubated with an
antigen that is specific to a patient's tumor, along with other compounds to
activate and mature the dendritic cells. These "programmed" cells are
then injected back into the bloodstream with the hopes that they will travel to
the lymph nodes and present the tumor antigen to the rest of the immune system
cells.
Biomaterials boost
immunity
While this approach
has had some clinical success, in most cases, the immune response resulting
from dendritic cell vaccines is short-lived and not robust enough to keep
tumors at bay over the long run. In addition, cell therapies such as this,
which require removing cells from patients and manipulating them in the lab,
are costly and not easily regulated. To overcome these limitations, Mooney's
lab has been experimenting with a newer approach that involves reprogramming
immune cells from inside the body using implantable biomaterials.
The idea is to
introduce a biodegradable scaffold under the skin that temporarily creates an
"infection-mimicking microenvironment," capable of attracting,
housing, and reprogramming millions of dendritic cells over a period of several
weeks. In a 2009 paper published in Nature Materials, Mooney demonstrated that
this could be achieved by loading a porous scaffold -- about the size of a dime
-- with tumor antigen as well as a combination of biological and chemical
components meant to attract and activate dendritic cells. Once implanted, the
scaffold's contents slowly diffused outward, recruiting a steady stream of
dendritic cells, which temporarily sought residence inside the scaffold while
being simultaneously exposed to tumor antigen and activating factors.
When the scaffold was
implanted in mice, it achieved a 90% survival rate in animals that otherwise
die from cancer within 25 days.
An injectable scaffold
Now, Mooney and his
team have taken this approach a step further, creating an injectable scaffold
that can spontaneously assemble once inside the body. This second generation vaccine
would prevent patients from having to undergo surgery to implant the scaffold
and would also make it easier for clinicians to administer it.
The new 3D vaccine is
made up of many microsized, porous silica rods dispersed in liquid. When
injected under the skin, the liquid quickly diffuses, leaving the rods behind
to form a randomly assembled three-dimensional structure resembling a haystack.
The spaces in between the rods are large enough to house dendritic cells and
other immune cells, and the rods have nano-sized pores that can be loaded with
a combination of antigens and drugs.
When injected into
mice that were then given a subsequent injection of lymphoma cells, the 3D
vaccine generated a potent immune response and delayed tumor growth. Compared
to a bolus injection containing the same drugs and antigens (but no scaffold),
the 3D vaccine was more effective at preventing tumor growth, with 90% of mice
receiving the 3D vaccine still alive at 30 days compared with only 60% of mice
given the bolus injection.
While the 3D
injectable scaffold is being tested in mice as a potential cancer vaccine, any
combination of different antigens and drugs could be loaded into the scaffold,
meaning it could also be used to treat infectious diseases that may be
resistant to conventional treatments.
"The ability to
so elegantly harness the natural behavior of dendritic cells to elicit a strong
immune response is impressive," says Jessica Tucker, Program Director of
Drug and Gene Delivery Systems and Devices at NIBIB. "The possibility of
developing this approach as a cancer vaccine, which would not require an
invasive and costly surgery to manipulate immune cells outside of the body, is
very exciting."
Mooney says that in
addition to continuing to develop the cancer vaccine, he also plans to explore
how the injectable scaffold can be used to both treat and prevent infectious
diseases. More broadly, Mooney predicts that spontaneously assembling particles
will be adopted by many fields in the future.
"I think this is
going to be the first of a number of examples where we utilize ideas of
self-organization in the body instead of having to create structures outside of
the body and place them in," says Mooney. "I think that will be
broadly applicable, not only in instances like this, but also, for example, in
tissue engineering and regenerative medicine where scaffolds are used to
facilitate the regrowth of tissues in the body. The ability to assemble a
scaffold in the body instead of having to surgically implant it would be a significant
advance."
Story Source:
The above story is
based on materials provided by National Institute of Biomedical Imaging
and Bioengineering. Note: Materials may be edited for
content and length.
Journal Reference:
1. Jaeyun Kim, Weiwei Aileen Li, Youngjin Choi,
Sarah A Lewin, Catia S Verbeke, Glenn Dranoff, David J Mooney. Injectable,
spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and
increase vaccine efficacy. Nature Biotechnology, 2014; 33 (1):
64 DOI: 10.1038/nbt.3071
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