Saturday, May 12, 2012

Gelatine Genetic Memory

Gelatine Genetic Memory.
Andre Willers
12 May 2012
“She turns my knees to jelly” popular song
Gelatine contains random fragments of the genotype’s history . These interact with the immune system to challenge it .
Discussion :
Collagen is a triple wound strand of molecules . It is a remnant of pre-RNA evolution , but too stable to be suitable for genetic information transmission . Instead it was adapted as a stable connective material (like rope) , twisted and knotted in stable and strong configurations . But it still contains coding sequences that the equally old immune system recognizes .
Gelatin is what remains after collagen is randomly broken into pieces and double strands by hydrolysis . It still contains genetic information from it’s RNA roots . There is also information from newer genetic material sticking to it .
In other words, gelatine can and is a better method of introducing foreign genetic material into a cell
Sigh . Yet another way of transmitting phenotype changes into the genotype . But much smoother than viruses or prions .
This has obvious application to auto-imune diseases . Eat jelly .
For example :
“Type II Collagen and Rheumatoid Arthritis
According to a study[42] published in the journal Science, oral administration of type II collagenimproves symptoms of rheumatoid arthritis. The authors conducted a randomized, double-blind trial involving 60 patients with severe, active rheumatoid arthritis. A decrease in the number of swollen joints and tender joints occurred in subjects fed with chicken type II collagen for 3 months, but not in those that received a placebo. Four patients in the collagen group had complete remission of the disease. No side effects were evident.”

You see . Chicken soup is good for you . It activates the immune system and keeps it out of mischief .

Optimizing gelatins .
This seems difficult , but it is analogeous to crumpling paper . See Appendix I .
You can use this to Calculate exactly the frequency of random codons remaining .
Work done by Dr Johan Willers indicates that repeated heating and cooling of gelatines (reformatting) increases their resistance to outside infection by many orders of magnitude . So , apart from the immunological response , there is another factor at play .
This is theorized to be hibernation triggering .Enough H2S is released that it triggers hibernation in the muscle cells .Part of the feedback process .(only about 80 ppm is needed) See appendix II
Gelatines (especially toughened ones) can then be used to target groups of auto-immune diseases .
Targeted H2S releases can render malefic organisms dormant , allowing the immune system to deal with them .
The random fragments will give the immune system something to chew on .
So , give your child jelly .Why do you think hospitals always give it ? It is an immune booster , especially in children .

The alert reader would have noticed that jelly is then a substitute for the Appendix Veriformis . Gelatine (especially if reheated many times) , will make a good substitute for an immune system on training wheels .

Will this work for Diabetes ?
Maybe type I , but Type II might need a bit more work . But I am definitely going to try it .

Radiation Damage :
A melange of high H2S and immune system activation fragments should protect against radiation Damage by a factor between 100 and 1000 .

Packing transplant organs in multiple-recast gelatines should prolong .
Packing burn patients in multiple-recast gelatins should prevent infections ..
But who cares about saving lives ? You can look decades younger by plastering multiply-recast gelatins across your face . It will work . Think it through for yourself .

Bah .
From your friendly Local Civilization .

Appendix I
Crumpling Paper and Space-Time
Andre Willers
23 Feb 2012

“The moving finger writes , and having writ , crumples it in random ruins.”
With apologies to Omar Khayyam .

Crumpled paper gives a good approximation of spacetime as a membrane with clumpy masses .
“Empty” spaces not occupied by the membrane gives an impression of dark matter .
We derive an expression to give this ratio using Infinite Descent and Beth(0) Random Walk .

Discussion :
1.The Crumpled paper :
Consider a paper disk of radius r and thickness d .
It's volume is then Vp=pi * r^2 * d
Draw a line from the center to the edge , in steps of length d , over the edge , then back to the center Let nu=r/d , a measure of the thickness of the paper . Note that it is a pure number .

The number of steps in the line is then n0=(2r/d)+1
But the number of steps to the edge of the original paper disk is n1=r/d=(n0-1)/2

Vp=(pi*d^3*(n0-1)^2 )/4

Crumple it up in a way that is as random as flipping a coin (ie Beth(0) )

The Trick : The line we have drawn up above breaks up into random vectors by rotating through a third dimension = crumpling into a ball .

We thus have a continuous line of random steps of known number of steps .
In 3 dimensions , the mean square distance from the center then is known
R = d * (n0)^0.5 …. See true for all dimensions as long as all are of Beth(0) order of randomness.

Volume of crumpled ball Vb=4/3*pi*R^3
The Ratio Vb/Vp = mu then gives the ratio of crumpled ball space to volume of paper mass .

Mu={4/3*pi*d^3 *n0^(3/2)} / (pi*d^3*(n0-1)^2 )/4
Notice the d^3 term and pi cancels out . This has profound physical implications .
This simplifies to

Expressed as thickness of paper , nu , which is a pure number independent of metric chosen .
mu=4*4/3(2*nu+1)^3/2 / (2*nu)^2
mu=4/3*(2nu+1)^3/2 / nu^2
This gives a quartic equation in nu , which can be solved exactly algebraically .
(mu)^2*(nu)*4 – 2^7/3^2 *(nu)^3 – 2^6/3^2 (nu)^2 – 2^5/3^2 * (nu)^1 - 2^4/3^2 =0

Test it on A4 paper:
A4 paper has thickness d~0,1 mm and r~150 mm
mu= 1- 0.90257841
This means that the crumpled A4 paper ball encloses about 90% empty space .
This agrees with experimental results . See NewScientist.

Note that the force applied does not matter . As long as the paper is untorn , mu will be the same .

How many times can it be folded ?
Solving the above (see below) gives mu=1 for about nu=14.7 to 14.8 .
This means there are no empty spaces left to fold into .

This can get complicated , so I will keep it simple .
Take a piece of paper and fold it . You then have a new piece of paper .The test-circle of same r will have double the thickness .
Ie , nu will double .
Between 7 and 8 folds , nu will hit the ceiling of mu=1 , regardless of the starting value of nu .
This is the maximum number of paper folds , as confirmed from other sources .

Physical interpretations :
Take an m-dimensional space . Randomness of order Beth(0) applies equally to all . The underlying equalizer . Collapse it to three dimensions and let the third one approach single Planck lengths .
Then we can use the above paper approximation . Notice how d cancels out except for an addition of 1 in final ratio .

What does it mean ?
See the physical universe as a brane (ie sheet of paper) in a multiverse . Crumpling it means it has mass and singularities . Both are aspects of the same thing .
An estimate of the number of singularities can be made from edges and points in crumpled paper .

Can we crumple the paper to a ball that is just paper ?
That is a particle .
The answer is “Yes” .

Such crumpling means that mu=1 (no empty space in any dimension )
This gives an quartic equation in nu that solves to four values , other dimensions than three denoted by i=(-1)^0.5

See for a calculator
nu1= 14.722181 (this makes the physical particle universe possible . Mass .
Nu2= - 0.004167 + i*0.49558 (Rotation :Spin :charge and magnetism)
nu3 = - 0.004167 - i*0.49558 (Rotation :Spin :charge and magnetism) notice the minus sign .
Nu4= - 0.49164542 (quantum effects as the particles dither. Inertia?)

What does a negative nu mean ?
nu=r/d . A negative nu means one of r or d must be negative .
1.If r is negative , it can be interpreted as curled up dimensions , inside the “outside” dimensions as defined by i . See “ The inside of zero” Aug 2009
2.If d is negative , it can be interpreted as quantum effects . A particle does not “occupy” all the space . Likes hopscotch .
3.But notice the the two are interrelated .The notorious observer effect . Where we place the minus sign between r or d .

There should be relationships between nu2 , nu3 and nu4 . Various rotations between macro- and micro dimensions .
This means the contraption is not symmetrical But we already know that ,

Physical constants :
Things like charge , mass , etc should be derivable from these basics . Hint:use lots of crumpled paper .

There is hope . The fact that it is quartic equation , which is always solvable , means that the Universe can be understood . Complicated and perverse , but as long as you stick to Beth(0) randomness , it can be understood . For higher orders of randomness , good luck .

Dark Matter :
I nearly forgot . Using Planck units , we can define the ratio of thickness of the brane as
nu=c*PlanckTime/(1*Planck Time)
nu=c = 3*10^8
This gives a
Mu=4/3*(2c+1)^3/2 / c^2
Simplifying (c is very large) . This gives the approximation
mu=4/3* 2^1.5 / c^0.5
mu=2.1773242 * 10^ (-4)
mu = 1-0.999783357
This means that 99.9783357 % of the universe can be interpreted as being “Dark Matter”.
Ie with attractive and repulsive qualities . Basically empty space .
May you have joy of that .

An interesting aside :Creative artists .
How many pieces of paper does an artist need to crumple up and throw away before he finds something acceptable ?
Something acceptable would translate to mu=1 . Thus , we can say 7-8 truly random foldings should give a result .
The same holds for cryptanalysis or any attempt to find an unknown .
Algorithm :
Try 8 times , crumple , then put it aside and try again later .
There is a quantum connection , strange as it might seem .

And what about a nice little Crumpling App for smartphones ?
But the randomness should be from truly random tables , not pseudo-random generators .

Randomly yours.

Hibernation and Cryogenics
Andre Willers
19 Nov 2008

Discussion :
Jessica Palmer pointed out on the 16th Nov 2008 that the primary problem with cryogenics seems to be in the re-establishment of metabolic processes after un-freezing .

But we have an already existing template for doing exactly that .

Hibernation .

See Appendix A

The evolutionary explanation is that during the transition-phase from methane/sulfur to oxygen atmosphere (circa 1.5 bn years ago) , there was a major advantage in suspending oxygen-driven systems if the organism found itself in a non-oxygen environment . It went into hibernation .

At the same time , alcohol was being excreted as a poison (like oxygen) . The ancestors of mitochondria (who could use low concentrations of oxygen or alcohol ) sought refuge in cells whose cell-walls were resistant (but not impervious) to alcohol transition .

Remember , alcohol is completely soluble in water , but oxygen is not . This difference drove the process . Alcohol concentrations in water could grow large , but oxygen-concentrations could not .

Mitochondria earned their keep by mopping up alcohol first and later converting oxygen to ATP . (The glucose and ketone metabolism came after this) . The ketone metabolism has never been very popular , because of the high loss-rate in excretory products , but has been kept as a third string on the bow . (Utilization of fat and protein-muscle reserves during low-glucose periods. )

Mitochondria thus has an exclusive preference of usage : alcohol , glucose , ketones in that order .

From experimental evidence (see Appendix A) , there is a genetic switch sensitive to the concentration of H2S to bring both the host cell and the mitochondrium to a state where all programmed molecular activity is suspended . (The power is switched off) .
But , of course , random beth(0) molecular activity due to temperature does not cease . Uncontrolled and anaerobic reactions still occur .

We get rid of most anaerobic organisms first .
Lots of sulfur , VitC and alcohol (fermented berries or carbohydrates in the stomach . A low acidity is required in the run-up to hibernation)
Then freeze .

Starting the contraption up again is a bit of a problem .

1. The power-plant :
The mitochondria needs to be primed with their preferred fuel (alcohol)
Oxygen needs to be infused .(Hyperbaric chamber)

2. Garbage disposal
The cellular garbage-disposal systems need to be activated . ATP from the powerplant needs to be allocated to breakdown-product disposal before the ATP is allocated to DNA/RNA production processes .

The garbage-disposal uses mechanisms that use sulfur to create the various vacuoles and ropes (cf mitosis) . Enough sulfur is vital .

Once again , oxygen and alcohol is used . Both are recognized by all systems as poisons to be removed as a first priority . They activate a quite sophisticated garbage-disposal system as H2S concentrations decrease .

3 . Flushing
All that garbage has to flushed away , preferably not through the kidneys or liver .
Use machines .
As H2S concentrations decrease , damage might occur due to PH fluctuations . Acidity (H2SO4 , etc) Buffering would be advisable .

4. Temperature:
Lots of water at 105 to 107 Fahrenheit for mammals , pulsing at pulserate(about 90 cycles per minute .)
This is to activate the chaperone systems and discourage opportunistic viruses .

5. Music
See “Music”
Play harmonious music so the vibrations can be felt throughout organism being thawed . This enhances timing-procedures by orders of magnitudes . Emergent order .
(A Beth(0.x) effect . )

The de-hibernization process must have an exact program at molecular level to reboot the cellular metabolism . Precisely what you need after a cryogenic procedure .
But its efficiency (ie your chance of survival) can be boosted by orders of magnitude by using the steps above .

Interesting notes:
1. Do hibernating animals like bears use alcohol-producing cells in their bloodstream to time hibernation? This can be tested .
2. Are there cold-chaperone molecules ? There should be .
3. Hibernation is easy . Nature has done all the hard work . Keeping the mechanism ticking over at a very slow rate enables cellular-garbage clearing for a relatively short period (6-8 months)
4. De-cryogenics is a bit harder , Beth(1) intervention is needed .
5. Alcohol-concentrations : we are talking about 1% to 2% imbibing . About 0.06% inside the cell . Ie , the cell-wall protects by a factor of about 30
6. Pulse-Cryogenics : alternate freezing and hibernation to get a better survival factor . For those who are too stupid to design a zero-entropy system .
Try : Life=negative entropy . Non-life = positive entropy . Design it so the sum is zero .



Appendix A
From “ Birdflu Update-4” dated 29 Oct 2005
Suspended Animation (the real thing!)
In 2005, Mark Roth and other scientists from the University of Washington and the Fred Hutchinson Cancer Research Center in Seattle demonstrated that mice can be put into a state of suspended animation by applying a low dosage of hydrogen sulfide (80 ppm H2S) in the air. The breathing rate of the animals sank from 120 to 10 breaths per minute and their temperature fell from 37 °C to 2 °C above ambient temperature (in effect, they had become cold-blooded). The mice survived this procedure for 6 hours and afterwards showed no negative health consequences.
Such a hibernation occurs naturally in many mammals and also in toads, but not in mice. (Mice can fall into a state called clinical torpor when food shortage occurs). If the H2S-induced hibernation can be made to work in humans, it could be useful in the emergency management of severely injured patients, and in the conservation of donated organs.
As mentioned above, hydrogen sulfide binds to cytochrome oxidase and thereby prevents oxygen from binding, which apparently leads to the dramatic slowdown of metabolism. Animals and humans naturally produce some hydrogen sulfide in their body; researchers have proposed that the gas is used to regulate metabolic activity and body temperature, which would explain the above findings
Dosages of H2S:
Treatment involves immediate inhalation of amyl nitrite, injections of sodium nitrite, inhalation of pure oxygen, administration of bronchodilators to overcome eventual bronchospasm, and in some cases hyperbaric oxygen therapy.
Exposure to lower concentrations can result in eye irritation (because of the high alkality of the SH- anion), a sore throat and cough, shortness of breath, and fluid in the lungs. These symptoms usually go away in a few weeks. Long-term, low-level exposure may result in fatigue, loss of appetite, headaches, irritability, poor memory, and dizziness. Higher concentrations of 700-800 ppm tend to be fatal.
• 0.0047 ppm is the recognition threshold, the concentration at which 50% of humans can detect the characteristic rotten egg odor of hydrogen sulfide [2]
• 10-20 ppm is the borderline concentration for eye irritation.
• 50-100 ppm leads to eye damage.
• At 150-250 ppm the olfactory nerve is paralyzed after a few inhalations, and the sense of smell disappears, often together with awareness of danger,
• 320-530 ppm leads to pulmonary edema with the possibility of death.
• 530-1000 ppm causes strong stimulation of the central nervous system and rapid breathing, leading to loss of breathing;
o 800 ppm is the lethal concentration for 50% of humans for 5 minutes exposition (LC50).
Concentrations over 1000 ppm cause immediate collapse with loss of breathing, even after inhalation of a single breath.


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